Publikacje
Effect of culture parameters on selenium accumulation in Saccharomyces cerevisiae cells
„Szulc-Musioł B., Dolińka B., Ryszka F. 2019. Effect of culture parameters on selenium accumulation in Saccharomyces cerevisiae cells. J. Elem., 24(1): 155 – 164. DOI: 10.5601/jelem.2018.23.2.1574”
DOI: 10.5601/jelem.2018.23.2.1574
Selenium may play a beneficial role in multi-factorial diseases with genetic and environmental linkages via regulation of the activity of selenoproteins. The supply of food supplemented with Se-enriched yeast is one of the ways to overcome deficiency of this microelement. The effect of incubation conditions on selenium binding by Saccharomyces cerevisiae was studied. The study process factors were as follows: kind of sugar (glucose, sucrose, fructose, lactose, maltose) and selenium concentration (0.1, 0.3, 0.7·10-3 mol dm-3). The cultures were incubated for 60 minutes in a shaker incubator at a temp. 30°C. The content of selenium in the yeast was determined by the fluorometric method. It was demonstrated that the yeast intracellular selenium concentration increased and the yeast dry biomass yield decreased along with an increased selenium concentration in the culture medium. The highest biomass production was obtained in the media containing glucose, while the lowest was in the media containing lactose; depending on the conditions of incubation, the accumulation of selenium into the yeast cells was in the range of 54.29–692.88 mg kg-1 of dry biomass. The highest amount of selenium, that is 692.88 mg kg-1 of dry biomass, was bound from the medium by baker’s yeast cultured in the presence of glucose and 0.7·10-3 mol dm-3 concentration of selenium. Under these cultivation conditions, almost no-waste yeast production was obtained – with the lowest amount (about 11.53%) of the remaining, i.e. unincorporated, selenium in the culture medium.
Słowa kluczowe: yeast; Saccharomyces cerevisiae; selenium; cultivation conditions; biomass yield
